B-066 Evaluation on Effectiveness of Colloidal Gold Immunochromatography Assay for Rapid Detection of Carbapenemase-producing <i>Enterobacteriaceae</i>

Abstract Background The identification, treatment, and control of carbapenem-resistant Enterobacteriaceae (CRE) infections are a major challenge for health care institutions diagnostic laboratories worldwide. A novel Carbapenemase-producing Bacteria Test Kit (Lateral Flow Assay) from Dynamiker Biotechnology (Tianjin) Co., Ltd. is qualitative test that was easy to operate can be widely accepted by clinical primary medical. Results read in just 15 minutes using colloidal gold immunochromatography, which detect double multiple carbapenemase types (Fig. 1). We analyzed the effectiveness carbapenemase-producing bacteria kit rapid detection CRE carbapenemase. Methods total 120 strains Enterobacterales blood culture were collected, including 80 40 CSE (carbapenem-susceptibility Enterobacteriaceae). PCR used KPC, NDM, IMP, VIM OXA-48 genes as standard. produced CRE, conducted performance evaluation combined with results. expressing all positive carbapenemasstre kit, includes 39 producing 26 8 5 2 enzymes; negative no detected. Compared results, sensitivity specificity four enzymes both 100% 2). Conclusion applied simple, rapid, sensitive specific method different types, great value accurate use anti-infective therapy.Fig. 1.Operating procedures immunochromatography.Fig. 2.Results C: Control, K: N: NMD, I: V: VIM, O: OXA-48.